U.S. Department of Energy

Pacific Northwest National Laboratory

Jordan Smith

Jordan Smith
Professional Title: 

Jordan Ned Smith, PhD, is a biomedical scientist at the Pacific Northwest National Laboratory (PNNL) focusing on biological fate and implications of chemical exposures on human health.  He has over 10 years of research experience investigating target-tissue dosimetry and associated biological response.  His research uses a combined experimental and computational approach to understand how (absorption, distribution, metabolism, and excretion (ADME) processes affect target-tissue dosimetry and mediate toxicity.  Dr. Smith has utilized this approach to understand pharmacokinetics and toxicity for a variety of compounds including pesticides, polycyclic aromatic hydrocarbons (PAHs), nanoparticles, organic esters, nicotine, and explosives.  Dr. Smith has extensive experience developing, reviewing, and applying computational tools for exposure assessment, tissue dose, and toxicology.

  • PhD Environmental Toxicology, Texas Tech University
  • BS Animal Science, University of Idaho
  • BS Veterinary Science, University of Idaho
  • Society of Toxicology (SOT) Biological Modeling Specialty Section Best Paper Published in 2017 Honorable Mention for “Predicting transport of 3,5,6-trichloro-2-pyridinol (TCPy) into saliva using a combination experimental and computational approach”.
  • Top 10 Abstract in SOT Risk Assessment Specialty Section for “Extrapolating Salivary Acinar Cell In Vitro Pesticide Transport to Whole Animals Using Computational Modeling”, at SOT Annual Meeting New Orleans, LA, USA (2016)
  • SOT’s Risk Assessment Specialty Section Award for Best Papers Published in 2013 Demonstrating Application of Risk Assessment for “A Multi-Route Model of Nicotine-Continine Pharmacokinetics, Pharmacodynamics, and Bain Nicotine Acetylcholine Receptor Binding in Humans”
  • SOT’s Best Postdoctoral Publication Award for “Pharmacokinetics of the chlorpyrifos metabolite 3,5,6-trichloro-2-pyridinol (TCPy) in rat saliva” (2011)
  • U.S. Department of Energy (DOE) Office of Science Undergraduate Research Programs Outstanding Mentor Award (2010)


  • Poet TS, C Timchalk, MJ Bartels, JN Smith, R Mcdougal, D Juberg, and PS Price. 2017. "Use of a probabilistic PBPK/PD model to calculate Data Derived Extrapolation Factors for chlorpyrifos." Regulatory Toxicology and Pharmacology 86:59-73. doi:10.1016/j.yrtph.2017.02.014
  • Smith JN, D Mehinagic, S Nag, SR Crowell, and RA Corley. 2017. "In vitro metabolism of benzo[a]pyrene-7,8-dihydrodiol and dibenzo[def,p]chrysene-11,12 diol in rodent and human hepatic microsomes." Toxicology Letters 269:23-32.  doi:10.1016/j.toxlet.2017.01.008
  • Smith JN, ZA Carver, TJ Weber, and C Timchalk. 2017. "Predicting Transport of 3,5,6-Trichloro-2-pyridinol into Saliva Using a Combination Experimental and Computational Approach." Toxicological Sciences 157(2):438-450.  doi:10.1093/toxsci/kfx055
  • Smith JN, KJ Tyrrell, JR Hansen, DG Thomas, TA Murphree, AK Shukla, T Luders, JM Madden, Y Li, AT Wright, and PD Piehowski. 2017. "Plasma Protein Turnover Rates in Rats Using Stable Isotope Labeling, Global Proteomics, and Activity-Based Protein Profiling." Analytical Chemistry 89(24):13559-13566.  doi:10.1021/acs.analchem.7b03984
  • Stoddard EG, BJ Killinger, RN Nair, NC Sadler, RF Volk, SO Purvine, AK Shukla, JN Smith, and AT Wright. 2017. "Activity-Based Probes for Isoenzyme- and Site-Specific Functional Characterization of Glutathione S- Transferases." Journal of the American Chemical Society 139(45):16032-16035.  doi:10.1021/jacs.7b07378
  • Timchalk C, TJ Weber, and JN Smith. 2017. "The Need for Non- or Minimally-Invasive Biomonitoring Strategies and the Development of Pharmacokinetic/Pharmacodynamic Models for Quantification." Current Opinion in Toxicology 4:28-34. doi:10.1016/j.cotox.2017.03.003
  • Wang Y, MM Zeinhom, M Yang, R Sun, S Wang, JN Smith, C Timchalk, L Li, Y Lin, and D Du. 2017. "A 3D-Printed, Portable, Optical-Sensing Platform for Smartphones Capable of detecting the Herbicide 2,4-Dichlorophenoxyacetic Acid." Analytical Chemistry 89(17):9339-9346.  doi:10.1021/acs.analchem.7b02139


  • Engelhard MH, JN Smith, and DR Baer. 2016. "Gold/silver Core-shell 20 nm Nanoparticles Extracted from Citrate Solution Examined by XPS." Surface Science Spectra 23(1):Article No. 29.  doi:10.1116/1.4953792
  • Sadler NC, P Nandhikonda, BJM Webb-Robertson, C Ansong, LN Anderson, JN Smith, RA Corley, and AT Wright. 2016. "Hepatic cytochrome P450 activity, abundance, and expression throughout human development." Drug Metabolism and Disposition 44(7):984-91. doi:10.1124/dmd.115.068593
  • Zhang X, MV Romm, X Zheng, EM Zink, YM Kim, KE Burnum-Johnson, DJ Orton, A Apffel, YM Ibrahim, ME Monroe, RJ Moore, JN Smith, J Ma, RS Renslow, DG Thomas, A Blackwell, G Swinford, J Sausen, RT Kurulugama Lekamlage, N Eno, E Darland, G Stafford, JC Fjeldsted, TO Metz, JG Teeguarden, RD Smith, and ES Baker. 2016. "SPE-IMS-MS: An automated platform for sub-sixty second surveillance of endogenous metabolites and xenobiotics in bio?uids." Clinical Mass Spectrometry 2:1-10.  doi:10.1016/j.clinms.2016.11.002


  • Mihai C, WB Chrisler, Y Xie, D Hu, CJ Szymanski, A Tolic, J Klein, JN Smith, BJ Tarasevich, and G Orr. 2015. "Intracellular accumulation dynamics and fate of zinc ions in alveolar epithelial cells exposed to airborne ZnO nanoparticles at the air-liquid interface." Nanotoxicology 9(1):9-22.  doi:10.3109/17435390.2013.859319
  • Munusamy P, CM Wang, MH Engelhard, DR Baer, JN Smith, C Liu, VK Kodali, BD Thrall, S Chen, PE Alexandra, and MP Ryan. 2015. "Comparison of 20 nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages." Biointerphases 10(3):031003-1 to 031003-16. doi:10.1116/1.4926547
  • Timchalk C, TJ Weber, and JN Smith. 2015. "Computational Strategy for Quantifying Human Pesticide Exposure based upon a Saliva Measurement." Frontiers in Pharmacology 6:Article No. 115.  doi:10.3389/fphar.2015.00115


  • Smith JN, PM Hinderliter, C Timchalk, MJ Bartels, and TS Poet. 2014. "A Human Life-Stage Physiologically Based Pharmacokinetic and Pharmacodynamic Model for Chlorpyrifos: Development and Validation." Regulatory Toxicology and Pharmacology 69(3):580-597. doi:10.1016/j.yrtph.2013.10.005


  • Smith JN. 2013. "Challenges for In vitro to in Vivo Extrapolation of Nanomaterial Dosimetry for Human Risk Assessment." Gradient EH&S Nano News 8(3):4. 
  • Teeguarden JG, C Housand, JN Smith, PM Hinderliter, R Gunawan, and C Timchalk. 2013. "A Multi-Route Model of Nicotine-Cotinine Pharmacokinetics, Pharmacodynamics and Brain Nicotinic Acetylcholine Receptor Binding in Humans." Regulatory Toxicology and Pharmacology 65(1):12-28.  doi:10.1016/J.YRTPH.2012.10.007
  • Lanekoff IT, M Thomas, JP Carson, JN Smith, C Timchalk, and J Laskin. 2013. "Imaging Nicotine in Rat Brain Tissue by Use of Nanospray Desorption Electrospray Ionization Mass Spectrometry." Analytical Chemistry 85(2):882-889.  doi:10.1021/ac302308p
  • Zhang W, Y Tang, D Du, JN Smith, C Timchalk, D Liu, and Y Lin. 2013. "Direct analysis of trichloropyridinol in human saliva using an Au nanoparticles-based immunochromatographic test strip for biomonitoring of exposure to chlorpyrifos." Talanta 114:261-267. doi:10.1016/j.talanta.2013.06.012


  • Smith JN, J Wang, Y Lin, EM Klohe, and C Timchalk. 2012. "Pharmacokinetics and Pharmacodynamics of Chlorpyrifos and 3,5,6-Trichloro-2-pyridinol in Rat Saliva After Chlorpyrifos Administration." Toxicological Sciences 130(2):245-256. doi:10.1093/toxsci/kfs251
  • Timchalk C, JN Smith, AL Hjerpe, TS Poet, and SK Lee. 2012. "Regional Brain Dosimetry for the Organophosphorus Insecticide Chlorpyrifos in the Preweanling Rat." Chapter 13 in Parameters for Pesticide QSAR and PBPK/PD Models for Human Risk Assessment. ACS Symposium Series, vol. 1099, ed. JB Knaak, C Timchalk and R Tornero-Velez, pp. 195-213.  American Chemical Society, Washington DC. 


  • Smith JN, C Timchalk, MJ Bartels, and TS Poet. 2011. "In Vitro Age-Dependent Enzymatic Metabolism of Chlorpyrifos and Chlorpyrifos-Oxon in Human Hepatic Microsomes and Chlorpyrifos-Oxon in Plasma." Drug Metabolism and Disposition 39(8):1353-1362. doi:10.1124/dmd.111.038745 
  • Du D, J Wang, L Wang, D Lu, JN Smith, C Timchalk, and Y Lin. 2011. "Magnetic Electrochemical Sensing Platform for Biomonitoring of Exposure to Organophosphorus Pesticides and Nerve Agents Based on Simultaneous Measurement of Total Enzyme Amount and Enzyme Activity ." Analytical Chemistry 83(10):3770-3777.  doi:10.1021/ac200217d
  • Ellison CA, JN Smith, PJ Lein, and JR Olson. 2011. "Pharmacokinetics and pharmacodynamics of chlorpyrifos in adult male Long-Evans rats following repeated subcutaneous exposure to chlorpyrifos." Toxicology 287(1-3):137-144. doi:10.1016/j.tox.2011.06.010
  • Lee SK, TS Poet, JN Smith, AL Hjerpe, R Gunawan, and C Timchalk. 2011. "IMPACT OF REPEATED NICOTINE AND ALCOHOL COEXPOSURE ON IN VITRO AND IN VIVO CHLORPYRIFOS DOSIMETRY AND CHOLINESTERASE INHIBITION." Journal of Toxicology and Environmental Health Part A 74(20):1334-50. 
  • Wang L, D Du, D Lu, CT Lin, JN Smith, C Timchalk, F Liu, J Wang, and Y Lin. 2011. "Enzyme-linked immunosorbent assay for detection of organophosphorylated butyrylcholinesterase: A biomarker of exposure to organophosphate agents." Analytica Chimica Acta 693(1-2):1-6. 
  • Wang L, D Lu, J Wang, D Du, Z Zou, H Wang, JN Smith, C Timchalk, F Liu, and Y Lin. 2011. "A Novel Immunochromatographic Electrochemical Biosensor for Highly Sensitive and Selective Detection of Trichloropyridinol, a Biomarker of Exposure to Chlorpyrifos." Biosensors and Bioelectronics 26(6):2835-2840 . doi:10.1016/j.bios.2010.11.008


  • Busby-Hjerpe AL, JA Campbell, JN Smith, S Lee, TS Poet, D Barr, and C Timchalk. 2010. "Comparative Pharmacokinetics of Chlorpyrifos versus its Major Metabolites Following Oral Administration in the Rat." Toxicology 268(1-2):55-63. doi:10.1016/j.tox.2009.11.022
  • Lee S, TS Poet, JN Smith, AL Busby-Hjerpe, and C Timchalk. 2010. "Effect of In Vivo Nicotine Exposure on Chlorpyrifos Pharmacokinetics and Pharmacodynamics in Rats ." Chemico-Biological Interactions 184(3):449-457. 
  • Lee SK, TS Poet, JN Smith, AL Busby-Hjerpe, and C Timchalk. 2010. "Effect of in vivo nicotine exposure on chlorpyrifos pharmacokinetics and pharmacodynamics in rats." Chemico-Biological Interactions 184(3):449-457.  doi:10.1016/j.cbi.2010.01.024
  • Smith JN, J Wang, Y Lin, and C Timchalk. 2010. "Pharmacokinetics of the Chlorpyrifos Metabolite 3,5,6-Trichloro-2-Pyridinol (TCPy) in Rat Saliva." Toxicological Sciences 132(2):315-325. 
  • Zou Z, D Du, J Wang, JN Smith, C Timchalk, YQ Li, and Y Lin. 2010. "Quantum Dot-Based Immunochromatographic Fluorescent Biosensor for Biomonitoring Trichloropyridinol, a Biomarker of Exposure to Chlorpyrifos." Analytical Chemistry 82(12):5125-5133. doi:10.1021/ac100260m


  • Du D, J Wang, JN Smith, C Timchalk, and Y Lin. 2009. "Biomonitoring of Organophosphorus Agent Exposure by Reactivation of Cholinesterase Enzyme Based on Carbon Nanotube-Enhanced Flow-Injection Amperometric Detection ." Analytical Chemistry 81(22):9314-9320. 
  • Smith JN, JA Campbell, AL Busby-Hjerpe, S Lee, TS Poet, D Barr, and C Timchalk. 2009. "Comparative chlorpyrifos pharmacokinetics via multiple routes of exposure and vehicles of administration in the adult rat." Toxicology 261(1-2):47-58. doi:10.1016/j.tox.2009.04.041
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