U.S. Department of Energy

Pacific Northwest National Laboratory

Jordan Smith

Jordan Smith
Professional Title: 
Biomedical Scientist

Jordan Ned Smith studies the biological fate of chemicals and implications of chemical exposures on human health.  Dr. Smith applies advanced molecular tools, cell-based models, in vivo techniques, and computational modeling to understand how absorption, distribution, metabolism, and excretion (ADME) processes affect dosimetry of chemicals and mediate toxicity.  His research contributions continue to expand our understanding of life-stage susceptibility to chemicals, non-invasive biomonitoring, target-tissue dosimetry, and biological fate of nanomaterials.

  • PhD Environmental Toxicology, Texas Tech University
  • BS Animal Science, University of Idaho
  • BS Veterinary Science, University of Idaho
  • Society of Toxicology (SOT) Biological Modeling Specialty Section Best Paper Published in 2019 Honorable Mention for “Physiologically based pharmacokinetic modeling of salivary concentrations for non-invasive biomonitoring of 2,4-dichlorophenoxyacetic acid (2,4-D)”.
  • Society of Toxicology (SOT) Biological Modeling Specialty Section Best Paper Published in 2017 Honorable Mention for “Predicting transport of 3,5,6-trichloro-2-pyridinol (TCPy) into saliva using a combination experimental and computational approach”.
  • Top 10 Abstract in SOT Risk Assessment Specialty Section for “Extrapolating Salivary Acinar Cell In Vitro Pesticide Transport to Whole Animals Using Computational Modeling”, at SOT Annual Meeting New Orleans, LA, USA (2016)
  • SOT’s Risk Assessment Specialty Section Award for Best Papers Published in 2013 Demonstrating Application of Risk Assessment for “A Multi-Route Model of Nicotine-Continine Pharmacokinetics, Pharmacodynamics, and Bain Nicotine Acetylcholine Receptor Binding in Humans”
  • SOT’s Best Postdoctoral Publication Award for “Pharmacokinetics of the chlorpyrifos metabolite 3,5,6-trichloro-2-pyridinol (TCPy) in rat saliva” (2011)
  • U.S. Department of Energy (DOE) Office of Science Undergraduate Research Programs Outstanding Mentor Award (2010)
Professional Memberships and Activities: 

Joint Appointment, Department of Environmental and Molecular Toxicology (EMT), Oregon State University

Member of Society of Toxicology (SOT)



  • Lin VS, Volk RF, DeLeon AJ, Anderson LN, Purvine SO, Shukla AK, Bernstein HC, Smith JN, Wright AT.  2020.  Structure Dependent Determination of Organophosphate Targets in Mammalian Tissues Using Activity-Based Protein Profiling. Chemical Research in Toxicology. 33: 414-425.  DOI: 10.1021/acs.chemrestox.9b00344.
  • Smith JN, Tyrrell KJ, Smith JP, Weitz KK, Faber W. 2020. Linking Internal Dosimetries of the Propyl Metabolic Series in Rats and Humans Using Physiologically Based Pharmacokinetic (PBPK) Modeling. Regulatory Toxicology and Pharmacology. 110:104507.  DOI: 10.1016/j.yrtph.2019.104507.


  • Han AA, Timchalk C, Carver ZA, Weber TJ, Tyrrell KJ, Sontag RL, Gibbins T, Chrisler WB, Weitz KK, Du A, Lin Y, Smith JN. 2019. Physiologically based pharmacokinetic modeling of salivary concentrations for non-invasive biomonitoring of 2,4-dichlorophenoxyacetic acid (2,4-D). Toxicological Sciences.  172(2): 330-343. DOI: 10.1093/toxsci/kfz206.  Note:  First published PBPK model using Magnolia software.
  • Niu X, Shi Q, Zhu W, Liu D, Tian H, Fu S, Cheng N, Li S, Smith JN, Du D, Lin Y.  2019.  Unprecedented Peroxidase-Mimicking Activity of Single-Atom Fe–Nx Moieties Hosted by MOF Derived Porous Carbon.  Biosensors & Bioelectronics. 142(1). 111495.  DOI: 10.1016/j.bios.2019.111495.
  • Weber TJ, Qian WJ, Smith JN, Gritsenko MA, Hu D, Chrisler WB, Timchalk C. Stable Acinar Progenitor Cell Model Identifies Treacle-Dependent Radioresistance.  Radiation Research.  Accepted.
  • Stoddard EG, Nag S, Martin J, Killinger BJ, Corley RA, Smith JN, and Wright AT. 2019. Benzo[a]pyrene Induction of Glutathione S-transferases: An activity-based protein profiling investigation. Chemical Research in Toxicology.  In Press. DOI: 10.1021/acs.chemrestox.9b00069.
  • Madeen EP, Siddens LK, Uesugi S, McQuistan T, Corley RA, Smith JN, Waters KM, Tilton SC, Anderson KA, Ognibene TJ, Turteltaub KW, Williams DE.  2019.  Toxicokinetics of Benzo[a]pyrene in Humans: Extensive metabolism as determined by UPLC-accelerator mass spectrometry following oral microdosing.  Toxicology and Applied Pharmacology. 364(1): 97–105. DOI: 10.1016/j.taap.2018.12.010.
  • Smith JN, Thomas DG, Jolley H, Kodali VK, Littke MH, Munusamy P, Baer D, Gaffrey MJ, Thrall BD, and Teeguarden JG.  2018.  All that is Silver is not Toxic: Silver Ion and Particle Kinetics Reveals the Role of Silver Ion Aging and Dosimetry on the Toxicity of Silver Nanoparticles.  Particle and Fibre Toxicology.  15(1):47. DOI: 10.1186/s12989-018-0283-z. PubMed PMID: 30518385.


  • Carver ZA, Han AA, Timchalk C, Weber TJ, Tyrrell KJ, Sontag RL, Luders T, Chrisler WB, Weitz KK, Smith JN.  2018.  Evaluation of Non-invasive Biomonitoring of 2,4-Dichlorophenoxyacetic Acid (2,4-D) in Saliva.  Toxicology.  410: 171-181. DOI: 10.1016/j.tox.2018.08.003.
  • Hummel JM, Madeen EP, Siddens LK, Uesugid S, McQuistand T, Anderson KA, Enright HA, Ogniben TJ, Bench G,  Kruegerd SK, Turteltaub KW, Harrish S, Smith JN, Tilton SC, Baird WM, Williams DE.  2018.  Pharmacokinetics of [14C]-benzo[a]pyrene (BaP) in humans: Impact of co-administration of smoked salmon and BaP dietary restriction.  Food and Chemical Toxicology. 115: 136-147.  DOI: 10.1016/j.fct.2018.03.003.
  • Melby ES, Yi C, Borgatta J, Mensch AC, Hang MN, Chrisler WB,Dohnalkova A, Van Gilder JM, Alvarez CM, Smith JN, Hamers RJ, Orr G. 2018. Impact of lithiated cobalt oxide and phosphate nanoparticles on rainbow trout gill epithelial cells.  Nanotoxicology. 19:1-16. DOI: 10.1080/17435390.2018.1508785.
  • Ouyang H, Lu Q, Wenwen W, Song Y, Luo L, Xinman T, Chengzhou Z, Smith JN, Du D, Fu Z, Lin Y.  2018.  Dual-readout immunochromatographic assay based on MnO2 nanoflowers-enhanced chemiluminescence.  Analytical Chemistry.  90(8): 5147-5152.  DOI: 10.1021/acs.analchem.7b05247.
  • Smith JN, Thomas DG, Jolley H, Kodali VK, Littke MH, Munusamy P, Baer D, Gaffrey MJ, Thrall BD, and Teeguarden JG.  2018.  All that is Silver is not Toxic: Silver Ion and Particle Kinetics Reveals the Role of Silver Ion Aging and Dosimetry on the Toxicity of Silver Nanoparticles.  Particle and Fibre Toxicology.  15(1):47. DOI: 10.1186/s12989-018-0283-z. PubMed PMID: 30518385.
  • Stoddard, EG, Volk RF, Carson JP, Ljungberg CM, Murphree TA, Smith JN, Sadler NC, Shukla AK, Ansong C, Wright AT.  Multifunctional Activity-Based Protein Profiling of the Developing Lung.  Journal of Proteome Research. 17(8) 2623-2634.  DOI: 10.1021/acs.jproteome.8b00086.
  • Thomas DG, Smith JN, Thrall BD, Baer DR, Jolley H, Prabhakaran M, Kodali V, Demokritou  P, Cohen J, Teeguarden JG.  2018.  ISD3: a particokinetic model for predicting the combined effects of particle sedimentation, diffusion and dissolution on cellular dosimetry for in vitro systems.  Particle and Fibre Toxicology.  15(6): 1-22.  DOI: 10.1186/s12989-018-0243-7.
  • Zhu Y, Dou M, Piehowski PD, Liang Y, Wang F, Chu RK, Chrisler WB, Smith JN, Schwarz KC, Shen Y, Shukla AK, Moore RJ, Smith RD, Qian W, Kelly RT. 2018. Spatially resolved proteome mapping of laser capture microdissected tissue with automated sample transfer to nanodroplets.  Molecular & Cellular Proteomics.  17(9) 1864-1874.  DOI: 10.1074/mcp.TIR118.000686.


  • Poet TS, C Timchalk, MJ Bartels, JN Smith, R Mcdougal, D Juberg, and PS Price. 2017. "Use of a probabilistic PBPK/PD model to calculate Data Derived Extrapolation Factors for chlorpyrifos." Regulatory Toxicology and Pharmacology 86:59-73. doi:10.1016/j.yrtph.2017.02.014
  • Smith JN, D Mehinagic, S Nag, SR Crowell, and RA Corley. 2017. "In vitro metabolism of benzo[a]pyrene-7,8-dihydrodiol and dibenzo[def,p]chrysene-11,12 diol in rodent and human hepatic microsomes." Toxicology Letters 269:23-32.  doi:10.1016/j.toxlet.2017.01.008
  • Smith JN, ZA Carver, TJ Weber, and C Timchalk. 2017. "Predicting Transport of 3,5,6-Trichloro-2-pyridinol into Saliva Using a Combination Experimental and Computational Approach." Toxicological Sciences 157(2):438-450.  doi:10.1093/toxsci/kfx055
  • Smith JN, KJ Tyrrell, JR Hansen, DG Thomas, TA Murphree, AK Shukla, T Luders, JM Madden, Y Li, AT Wright, and PD Piehowski. 2017. "Plasma Protein Turnover Rates in Rats Using Stable Isotope Labeling, Global Proteomics, and Activity-Based Protein Profiling." Analytical Chemistry 89(24):13559-13566.  doi:10.1021/acs.analchem.7b03984
  • Stoddard EG, BJ Killinger, RN Nair, NC Sadler, RF Volk, SO Purvine, AK Shukla, JN Smith, and AT Wright. 2017. "Activity-Based Probes for Isoenzyme- and Site-Specific Functional Characterization of Glutathione S- Transferases." Journal of the American Chemical Society 139(45):16032-16035.  doi:10.1021/jacs.7b07378
  • Timchalk C, TJ Weber, and JN Smith. 2017. "The Need for Non- or Minimally-Invasive Biomonitoring Strategies and the Development of Pharmacokinetic/Pharmacodynamic Models for Quantification." Current Opinion in Toxicology 4:28-34. doi:10.1016/j.cotox.2017.03.003
  • Wang Y, MM Zeinhom, M Yang, R Sun, S Wang, JN Smith, C Timchalk, L Li, Y Lin, and D Du. 2017. "A 3D-Printed, Portable, Optical-Sensing Platform for Smartphones Capable of detecting the Herbicide 2,4-Dichlorophenoxyacetic Acid." Analytical Chemistry 89(17):9339-9346.  doi:10.1021/acs.analchem.7b02139


  • Engelhard MH, JN Smith, and DR Baer. 2016. "Gold/silver Core-shell 20 nm Nanoparticles Extracted from Citrate Solution Examined by XPS." Surface Science Spectra 23(1):Article No. 29.  doi:10.1116/1.4953792
  • Sadler NC, P Nandhikonda, BJM Webb-Robertson, C Ansong, LN Anderson, JN Smith, RA Corley, and AT Wright. 2016. "Hepatic cytochrome P450 activity, abundance, and expression throughout human development." Drug Metabolism and Disposition 44(7):984-91. doi:10.1124/dmd.115.068593
  • Zhang X, MV Romm, X Zheng, EM Zink, YM Kim, KE Burnum-Johnson, DJ Orton, A Apffel, YM Ibrahim, ME Monroe, RJ Moore, JN Smith, J Ma, RS Renslow, DG Thomas, A Blackwell, G Swinford, J Sausen, RT Kurulugama Lekamlage, N Eno, E Darland, G Stafford, JC Fjeldsted, TO Metz, JG Teeguarden, RD Smith, and ES Baker. 2016. "SPE-IMS-MS: An automated platform for sub-sixty second surveillance of endogenous metabolites and xenobiotics in bio?uids." Clinical Mass Spectrometry 2:1-10.  doi:10.1016/j.clinms.2016.11.002


  • Mihai C, WB Chrisler, Y Xie, D Hu, CJ Szymanski, A Tolic, J Klein, JN Smith, BJ Tarasevich, and G Orr. 2015. "Intracellular accumulation dynamics and fate of zinc ions in alveolar epithelial cells exposed to airborne ZnO nanoparticles at the air-liquid interface." Nanotoxicology 9(1):9-22.  doi:10.3109/17435390.2013.859319
  • Munusamy P, CM Wang, MH Engelhard, DR Baer, JN Smith, C Liu, VK Kodali, BD Thrall, S Chen, PE Alexandra, and MP Ryan. 2015. "Comparison of 20 nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages." Biointerphases 10(3):031003-1 to 031003-16. doi:10.1116/1.4926547
  • Timchalk C, TJ Weber, and JN Smith. 2015. "Computational Strategy for Quantifying Human Pesticide Exposure based upon a Saliva Measurement." Frontiers in Pharmacology 6:Article No. 115.  doi:10.3389/fphar.2015.00115


  • Smith JN, PM Hinderliter, C Timchalk, MJ Bartels, and TS Poet. 2014. "A Human Life-Stage Physiologically Based Pharmacokinetic and Pharmacodynamic Model for Chlorpyrifos: Development and Validation." Regulatory Toxicology and Pharmacology 69(3):580-597. doi:10.1016/j.yrtph.2013.10.005


  • Smith JN. 2013. "Challenges for In vitro to in Vivo Extrapolation of Nanomaterial Dosimetry for Human Risk Assessment." Gradient EH&S Nano News 8(3):4. 
  • Teeguarden JG, C Housand, JN Smith, PM Hinderliter, R Gunawan, and C Timchalk. 2013. "A Multi-Route Model of Nicotine-Cotinine Pharmacokinetics, Pharmacodynamics and Brain Nicotinic Acetylcholine Receptor Binding in Humans." Regulatory Toxicology and Pharmacology 65(1):12-28.  doi:10.1016/J.YRTPH.2012.10.007
  • Lanekoff IT, M Thomas, JP Carson, JN Smith, C Timchalk, and J Laskin. 2013. "Imaging Nicotine in Rat Brain Tissue by Use of Nanospray Desorption Electrospray Ionization Mass Spectrometry." Analytical Chemistry 85(2):882-889.  doi:10.1021/ac302308p
  • Zhang W, Y Tang, D Du, JN Smith, C Timchalk, D Liu, and Y Lin. 2013. "Direct analysis of trichloropyridinol in human saliva using an Au nanoparticles-based immunochromatographic test strip for biomonitoring of exposure to chlorpyrifos." Talanta 114:261-267. doi:10.1016/j.talanta.2013.06.012


  • Smith JN, J Wang, Y Lin, EM Klohe, and C Timchalk. 2012. "Pharmacokinetics and Pharmacodynamics of Chlorpyrifos and 3,5,6-Trichloro-2-pyridinol in Rat Saliva After Chlorpyrifos Administration." Toxicological Sciences 130(2):245-256. doi:10.1093/toxsci/kfs251
  • Timchalk C, JN Smith, AL Hjerpe, TS Poet, and SK Lee. 2012. "Regional Brain Dosimetry for the Organophosphorus Insecticide Chlorpyrifos in the Preweanling Rat." Chapter 13 in Parameters for Pesticide QSAR and PBPK/PD Models for Human Risk Assessment. ACS Symposium Series, vol. 1099, ed. JB Knaak, C Timchalk and R Tornero-Velez, pp. 195-213.  American Chemical Society, Washington DC. 


  • Smith JN, C Timchalk, MJ Bartels, and TS Poet. 2011. "In Vitro Age-Dependent Enzymatic Metabolism of Chlorpyrifos and Chlorpyrifos-Oxon in Human Hepatic Microsomes and Chlorpyrifos-Oxon in Plasma." Drug Metabolism and Disposition 39(8):1353-1362. doi:10.1124/dmd.111.038745 
  • Du D, J Wang, L Wang, D Lu, JN Smith, C Timchalk, and Y Lin. 2011. "Magnetic Electrochemical Sensing Platform for Biomonitoring of Exposure to Organophosphorus Pesticides and Nerve Agents Based on Simultaneous Measurement of Total Enzyme Amount and Enzyme Activity ." Analytical Chemistry 83(10):3770-3777.  doi:10.1021/ac200217d
  • Ellison CA, JN Smith, PJ Lein, and JR Olson. 2011. "Pharmacokinetics and pharmacodynamics of chlorpyrifos in adult male Long-Evans rats following repeated subcutaneous exposure to chlorpyrifos." Toxicology 287(1-3):137-144. doi:10.1016/j.tox.2011.06.010
  • Lee S, Poet TS, Smith JN, Hjerpe AL, Gunawan R, Timchalk C. 2011. Impact of repeated nicotine and alcohol co-exposure on in vitro and in vivo chlorpyrifos dosimetry and cholinesterase inhibition. Journal of Toxicology and Environmental Health. Part A, 74:1334-50.  DOI: 10.1080/15287394.2011.567958. 
  • Wang L, D Du, D Lu, CT Lin, JN Smith, C Timchalk, F Liu, J Wang, and Y Lin. 2011. "Enzyme-linked immunosorbent assay for detection of organophosphorylated butyrylcholinesterase: A biomarker of exposure to organophosphate agents." Analytica Chimica Acta 693(1-2):1-6. 
  • Wang L, D Lu, J Wang, D Du, Z Zou, H Wang, JN Smith, C Timchalk, F Liu, and Y Lin. 2011. "A Novel Immunochromatographic Electrochemical Biosensor for Highly Sensitive and Selective Detection of Trichloropyridinol, a Biomarker of Exposure to Chlorpyrifos." Biosensors and Bioelectronics 26(6):2835-2840 . doi:10.1016/j.bios.2010.11.008


  • Busby-Hjerpe AL, JA Campbell, JN Smith, S Lee, TS Poet, D Barr, and C Timchalk. 2010. "Comparative Pharmacokinetics of Chlorpyrifos versus its Major Metabolites Following Oral Administration in the Rat." Toxicology 268(1-2):55-63. doi:10.1016/j.tox.2009.11.022
  • Lee S, TS Poet, JN Smith, AL Busby-Hjerpe, and C Timchalk. 2010. "Effect of In Vivo Nicotine Exposure on Chlorpyrifos Pharmacokinetics and Pharmacodynamics in Rats ." Chemico-Biological Interactions 184(3):449-457. 
  • Lee SK, TS Poet, JN Smith, AL Busby-Hjerpe, and C Timchalk. 2010. "Effect of in vivo nicotine exposure on chlorpyrifos pharmacokinetics and pharmacodynamics in rats." Chemico-Biological Interactions 184(3):449-457.  doi:10.1016/j.cbi.2010.01.024
  • Smith JN, J Wang, Y Lin, and C Timchalk. 2010. "Pharmacokinetics of the Chlorpyrifos Metabolite 3,5,6-Trichloro-2-Pyridinol (TCPy) in Rat Saliva." Toxicological Sciences 132(2):315-325. 
  • Zou Z, D Du, J Wang, JN Smith, C Timchalk, YQ Li, and Y Lin. 2010. "Quantum Dot-Based Immunochromatographic Fluorescent Biosensor for Biomonitoring Trichloropyridinol, a Biomarker of Exposure to Chlorpyrifos." Analytical Chemistry 82(12):5125-5133. doi:10.1021/ac100260m


  • Du D, J Wang, JN Smith, C Timchalk, and Y Lin. 2009. "Biomonitoring of Organophosphorus Agent Exposure by Reactivation of Cholinesterase Enzyme Based on Carbon Nanotube-Enhanced Flow-Injection Amperometric Detection ." Analytical Chemistry 81(22):9314-9320. 
  • Smith JN, JA Campbell, AL Busby-Hjerpe, S Lee, TS Poet, D Barr, and C Timchalk. 2009. "Comparative chlorpyrifos pharmacokinetics via multiple routes of exposure and vehicles of administration in the adult rat." Toxicology 261(1-2):47-58. doi:10.1016/j.tox.2009.04.041
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